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In summary the cell density
2018-10-20
In summary, the cell density of PDX1+ posterior foregut glutathione s-transferase positively correlates with the induction of PDX1+NKX6.1+ pancreatic bud cells in hESC/iPSC differentiation cultures. Although the molecules that regulate the differentiation step remain unknown, the signals elicited b
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Acquiring an enriched population of a given cell type using
2018-10-20
Acquiring an enriched population of a given cell type using a fluorescence-based reporter line has many advantages but requires that the reporter expression is specific to the cell type to be enriched. Glial fibrillary acidic protein (GFAP) is a well-known marker for astrocytes; its expression is dy
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CXCL has been previously identified as an estrogen regulated
2018-10-20
CXCL12 has been previously identified as an estrogen-regulated gene in estrogen receptor (ER)-positive ovarian and breast cancer niclosamide (Jaerve et al., 2012). Here we show that in the endometrium, E2 significantly increased CXCL12 expression, suggesting a mechanism by which stem cells are recr
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CXCL has been previously identified as an estrogen regulated
2018-10-20
CXCL12 has been previously identified as an estrogen-regulated gene in estrogen receptor (ER)-positive ovarian and breast cancer hydroxypropyl beta cyclodextrin (Jaerve et al., 2012). Here we show that in the endometrium, E2 significantly increased CXCL12 expression, suggesting a mechanism by which
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br Conclusion br Contributions br Competing interests br Fun
2018-10-20
Conclusion Contributions Competing interests Funding This study was financially supported by German Academic Exchange Service/Federal Ministry of Education and Research (grant # D/09/04774), Center for Regenerative Therapies Dresden (Seed Grant # 09-08), Simon Fougner Hartmanns Family Fo
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Using ESNPs to repair damaged tissue in neurodegenerative di
2018-10-20
Using ESNPs to repair damaged tissue in neurodegenerative diseases is an attractive option, as these buy XL184 are more readily obtained than fetal material. Recent work on the relationship between neural stem cells and blood vessels (reviewed in (Bjornsson et al., 2015) as well as our own transplan
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HSCs isolated by the set of markers which are
2018-10-20
HSCs isolated by the set of markers which are Lin−/Sca-1+/C-kit+ (LSK) have been also reported to be heterogeneous (Osawa et al., 1996). Moreover, Sca-1 expression differs between mouse strains and therefore is not very effective to enrich HSCs (Spangrude and Brooks, 1993). Likewise, the SLAM family
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The host immune response has
2018-10-20
The host immune response has been identified as a critical regulator of tumor development and progression (Dunn et al., 2004; Fridman et al., 2013; Hadrup et al., 2013). Several types of immune myeloperoxidase participate in immunosurveillance by direct killing or production of cytokines. Our expres
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Next to the significant evidence that CpG methylation of the
2018-10-20
Next to the significant evidence that CpG methylation of the HLA-G promoter is important for the transcriptional activity of the gene (Moreau et al., 2003; Mouillot et al., 2005), HLA-G leukotriene receptor antagonist is also post-transcriptionally regulated by the 3′UTR. Several polymorphic sites
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br Experimental Procedures br Author Contributions br Acknow
2018-10-20
Experimental Procedures Author Contributions Acknowledgments Introduction Intestinal crypts contain stem cells and their transit-amplifying (TA) daughter cells. Cells exiting the proliferative crypts onto the villi terminally differentiate into enterocytes, goblet cells, and enteroendocr
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br Experimental Procedures br Acknowledgments
2018-10-20
Experimental Procedures Acknowledgments Introduction The kidney contains multiple specialized cell types that have district physiological functions. During embryogenesis, the urogenital system, including the kidney, is derived from the intermediate mesoderm of the developing embryo (Herzlin
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The most significant finding in these studies was the fold
2018-10-20
The most significant finding in these studies was the 10-fold enhanced expansion of transplantable HSCs in mice engrafted with IK6-transduced human CD34+ CB cells. This was indicated both by the higher numbers of CD34+CD38− dihydrofolate reductase inhibitor antibacterial regenerated from the IK6-tr
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Main Text br Acknowledgments The
2018-10-20
Main Text Acknowledgments The authors would like to thank all delegates for their input into the workshops that led to the contents of this white paper (Table S2). Introduction The type II clustered regularly interspaced short palindromic repeat (CRISPR) systems and the associated Cas9 nucle
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To analyze whether the pB Tet GOI system
2018-10-20
To analyze whether the pB-Tet-GOI system expresses proteins that are biologically active and at sufficient levels to direct specific neuronal differentiation, HuNPCs were nucleofected with each response plasmid and differentiated for up to 6 weeks. Live gaba receptors were imaged daily for GFP and T
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Of importance was our finding of changes in other PD
2018-10-20
Of importance was our finding of changes in other PD-associated genes, including SNCA (Krüger et al., 1998; Polymeropoulos et al., 1997; Singleton et al., 2003; Zarranz et al., 2004). SNCA is a presynaptic protein and function in regulating synaptic vesicle and neurotransmitter release. Aggregates o
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