CCK Octapeptide, non-sulfated A previous study showed that m

A previous study showed that miR-200c is involved in glucose-mediated pathological processes (Zhang, Guan, & Jin, 2017). Glucose metabolism is critical for the growth and proliferation of both normal CCK Octapeptide, non-sulfated and cancer cells, and reprogramming glucose metabolism now has been considered as a target of the treatment of malignancies (Hay, 2016). In this study, overexpression of miR-200c significantly reduced glucose uptake of cells of both human oral squamous cell carcinoma cell lines. In addition, miR-200c overexpression also significantly inhibited the proliferation of cancer cells. Those data indicate that overexpression of miR-200c may serve as a potential target for the treatment of oral squamous cell carcinoma. Activation of Akt signaling is common in nearly all of the human malignancies (Altomare & Testa, 2005). It has been reported that microRNA-200c targets Akt signaling pathway to inhibit cell apoptosis of pituitary adenoma (Liao et al., 2014). In addition, Akt signaling pathway regulates the expression of Glut-1, which plays a key role in glucose metabolism (Lo et al., 2011). In this study, microRNA-200c overexpression significantly upregulated the expression level of Glut-1 and phosphorylation level of Akt in oral squamous cell carcinoma cells. However, Akt activator treatment and Glut-1 overexpression showed no significant effect on miR-200c. Besides that, treatment with Akt activator SC79 for 1 hour significantly increased the expression level of Glut-1 in both oral squamous cell carcinoma cell lines, but Glut-1 overexpression showed no significant effects on levels of Akt and p-Akt. Those data suggest that miR-200c is an upstream inhibitor of Akt pathway, which is an upstream of Glut-1 in the pathogenesis of oral squamous cell carcinoma.
Conclusion